Evaluation of the Lumac kit for the detection of bacteriuria by bioluminescence.
نویسندگان
چکیده
Four hundred and twenty-two urine samples were screened for significant bacteriuria using bioluminescence and microscopy of uncentrifuged urine. A smaller number of false-negatives were seen with bioluminescence (10%) than with microscopy (40%) while both techniques gave a similar number of false-positives (18%). The kit required a large amount of manual preparation, largely pipetting. With this and the short shelf-life of the reconstituted reagents, it is not suitable for small numbers of urines. At 45p per urine, the cost of bioluminescence is too high.
منابع مشابه
Comparison of the Lumac and Monolight systems for detection of bacteriuria by bioluminescence.
The development of practical and rapid methods for detection of infectious-disease-producing agents in clinical specimens is the most important current goal of clinical microbiology. Bioluminescence is a technique which is rapid and potentially sensitive enough to detect significant numbers of bacteria in urine specimens. To determine whether bioluminescence is practical and cost effective for ...
متن کاملClinical evaluation of the Lumac bioluminescence method for screening urine specimens.
A bioluminescence method for screening urine cultures to provide rapid reporting of negative specimens and to select appropriate urine cultures for direct application of automated identification methods was evaluated. A total of 2,000 specimens were processed in the Lumac Biocounter (3M, St. Paul, Minn.), and the results were compared with quantitative culture techniques by using a 0.001-ml ino...
متن کاملEvaluation of a nitrite test kit (Stat-test) for the detection of significant bacteriuria.
A commercially marketed nitrite test kit (Stat-test) for the rapid detection of significant bacteriuria has been evaluated and found to have failed to detect 50% of all cases of significant bacteriuria and 46% of significant bacteriuria associated with nitrate-reducing organisms. The number of false positive results are negligible. The Stat-test, used in its present form, cannot be recommended ...
متن کاملAnalysis of the disagreement between automated bioluminescence-based and culture methods for detecting significant bacteriuria, with proposals for standardizing evaluations of bacteriuria detection methods.
A fully automated method for detecting significant bacteriuria is described which uses firefly luciferin and luciferase to detect bacterial ATP in urine. The automated method was calibrated and evaluated, using 308 urine specimens, against two reference culture methods. We obtained a specificity of 0.79 and sensitivity of 0.75 using a quantitative pour plate reference test and a specificity of ...
متن کاملVisualization of Leishmania tropica Infection in BALB/c Mice by Bioluminescence Imaging
Background: Leishmania tropica is the cause of more than one form of leishmaniasis and lacks a known reservoir animal. This study compares the potential infectivity of recombinant and wild-type L. tropica in BALB/c mice. Methods: The potential infectivity of recombinant L. tropicaEGFP or L. tropicaEGFP-LUC by two different, the subcutaneous and intradermal, routes was compared using a range of ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of clinical pathology
دوره 35 1 شماره
صفحات -
تاریخ انتشار 1982